PCR实验室规划

"Amplification examination laboratory is generally divided into four separate working areas / 扩增检验实验室原则上分为四个单独的工作区域

reagent storage and preparation area, sample preparation area, amplification reaction mixture preparation and amplification area and amplified product analysis area. In order to avoid cross contamination, all working areas must be entered in a single direction.

试剂贮存和准备区、标本制备区、扩增反应混合物配制和扩增区、扩增产物分析区。为避免交叉污染，进入各个工作区域必须严格遵循单一方向进行。

Reagent preparation area→ sample preparation area → amplification area→product analysis area

试剂准备区→样品制备区→扩增区→产物分析区。

Reagents and samples between each experimental area should be passed through the window. 

各实验区之间的试剂及样品传递应通过传递窗进行。

Reagent preparation area / 试剂准备区  

The main operations of the reagent preparation area include preparation/packaging of reagents and main reaction mixture preparation. Reagents and materials used for sample production shall be directly transferred to this area without going through other areas. Reagent raw materials must be stored in this area and prepare the required storage reagents in this area. As for air pressure control, no strict requirements are required.

该实验区主要进行的操作为贮存试剂的制备、试剂的分装和主反应混合液的制备。试剂和用于样品制作的材料应直接运送至该区，不得经过其他区域。试剂原材料必须贮存在本区内，并在本区内制备成所需的贮存试剂。 对与气流压力的控制，本区并没有严格的要求。

Sample preparation area / 标品制备区  

The main operations in the area include sample preservation, nucleic acid (RNA, DNA) extraction, storage and addition to amplification reaction tube and determination of DNA synthesis. 

该区域主要进行的操作为样本的保存、核酸(RNA、DNA)提取、贮存及其加入至扩增反应管和测定DNA的合成。

The pressure gradient in this area is required to be positive pressure relative to the adjacent area.  in order to avoid aerosol pollution from the adjacent area. In addition, due to the pollution caused by aerosol in the sample operation, it is necessary to avoid unnecessary movement in this area.

本区的压力梯度要求为：相对于邻近区域为正压，以避免从邻近区进入本区的气溶胶污染。另外，由于在加样操作中可能会发生气溶胶所致的污染，所以应避免在本区内不必要的走动。

Amplification area / 扩增区  

The main operations in this area include DNA amplification. In addition, addition of prepared DNA template (from sample preparation area) and preparation of reaction mixture from main reaction mixture (from reagent storage and preparation area) can also be carried out in this area.

该区域主要进行的操作为DNA扩增。此外，已制备的DNA模板(来自样本制备区)的加入和主反应混合液(来自试剂贮存和制备区)制备成反应混合液等也可在本区内进行。

The pressure gradient in this area is required to be negative pressure relative to the adjacent area in order to avoid leakage of aerosol from this area. In order to avoid pollution caused by aerosol, it is necessary to avoid unnecessary movement in this area. Individual operation such as sample addition should be carried out on super clean table.

本区的压力梯度要求为：相对于邻近区域为负压，以避免气溶胶从本区漏出。为避免气溶胶所致的污染，应尽量减少在本区内的不必要的走动。个别操作如加样等应在超净台内进行。

Product analysis area / 产物分析区  

The main operations in this area include determination of amplified fragments.

该区域主要进行的操作为扩增片段的测定。

This area is the main source for amplification product contamination, so the pressure gradient in this area is required to be negative pressure relative to the adjacent area in order to avoid amplification product from passing from this area to other areas.

本区是主要的扩增产物污染来源，因此对本区的压力梯度的要求为：相对于邻近区域为负压，以避免扩增产物从本区扩散至其它区域。"